Description de l'Article
Amersham Biosciences MegaBACE 1000 Specifications MegaBACE 1000 DNA Analysis System is a high-throughput, fluorescence-based DNA system utilizing capillary electrophoresis with up to 96 capillaries operating in parallel. The system performs sample injection, gel matrix replacement, DNA separation, detection, and data analysis, offering unparralleled flexibility. Components Capillary electrophoresis instrument Up to 6 arrays of 16 capillaries/array for a total of 96 capillaries Separation matrix for DNA analysis Reagents for DNA sequencing and genotyping Instrument control and data analysis software Computer workstation and monitor for instrument operation and data analysis Capillary arrays The capillaries are supplied in pre-assembled arrays of 16 capillaries. 16, 32, 48, 64, 80 or 96 capillaries can be used during a run. 200 Mu outer diameter 75 Mu internal diameter 40 cm detection length Separation matrix MegaBACE Long Read Matrix is optimized for both sequencing and genotyping applications. This saves time when switching between applications, and results in less waste. MegaBACE Long Read Matrix can produce DNA sequences of 700 bases or resolve multiplexed fragments in excess of 900 bases. The gel matrix is replaceable, and each pack includes 96 pre-dispensed tubes plus running buffer. Sequencing reagents All MegaBACE DNA sequencing reagent kits offer unparalleled performance on a broad range of templates: DYEnamic ET Dye Terminators or DYEnamic ET Primers DYEnamic ET Primer Reagents Genotyping reagents To complement instrument functionality, we offer a range of MegaBACE ET Size Standards for a variety of applications. These include MegaBACE ET400-R, ET550-R, and ET900-R. For SNP validation, MegaBACE SNuPe Genotyping Kit contains everything needed to perform a robust single-base extension reaction of PCR products in a single-tube format. Software The following software tools are available: Instrument Control Manager is used for instrument set-up and sample entry. Sequence Analyzer processes large amounts of data without manual intervention. MegaBACE Sequencing ScoreCard offers a time-saving overview of all samples. MegaBACE Fragment Profiler allows the user to automate the analysis of di-, tri-, and tetranucleotide repeats of polyploid organisms. User optimized peak filters allow for the automated analysis of atypical peak patterns. MegaBACE Genetic Profiler is optimized for diploid dinucleotide repeat analysis. MegaBACE Genetic Profiler ScoreCard allows quality comparisons between capillaries and runs. MegaBACE SNP Profiler saves editing time by assigning quality scores to each SNP call. Computer requirements Hardware: Pentium4 2.4GHz Operating system: Windows 2000 Installed RAM: 512 MB or more Software: Microsoft Office Pro Specifications Sequencing read length and throughput With the ability to sequence 96 samples in just 2 hours and perform up to 9 runs per day, MegaBACE 1000 is the ideal choice for production-scale sequencing projects. Average read length with dye primer chemistry is 550 bp at 98.5% accuracy with M 13 standard template. With a slower, 3-hour run time, sequences of up to 1000 bases is possible. MegaBACE 1000 can produce more than 500 samples per 9-hour day. Genotyping sizing precision, accuracy, and throughput The MegaBACE DNA Genotyping System automatically processes 2000 microsatellite genotypes per 96-well run. The total turnaround time per run is less than 2 hours from injection to injection. This corresponds to more than 14 000 genotypes per system per day. For SNP analysis, MegaBACE 1000 can deliver >5 760 SNPs per day. Sample requirement Minimal sample loading volume of 5 Mul. The samples are introduced to the system in a 96-well format. Samples can be reinjected up to 10 times. Excitation Single mode (488 nm) for sequencing Dual mode (488 and 532 nm) for genotyping Detection 4 channels with 2 PMTs Confocal optics with multiple filter sets Digital resolution is 16 bits linear to 5% Electrophoresis Voltage: Up to 320 V/cm Chamber Operating Temperature: 27 deg C to 44 deg C Environmental Requirements Temperature: 20 deg C to 25 deg C Humidity: 80% noncondensing Weight: 272 kg (approximate) Dimensions: 81.2 cm (height) x 103.3 cm (width) x 87.4 cm (depth)
